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pTAMACHis6_PiuD
(Plasmid #128953)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128953 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTAMACHIS6
  • Backbone size w/o insert (bp) 3725
  • Total vector size (bp) 5866
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    protein expression in C43(DE3) strains
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Putative outer membrane receptor for iron transport (PiuD)
  • Alt name
    PiuD
  • Species
    P. aeruginosa strain 39016
  • Insert Size (bp)
    2193
  • Mutation
    Signal peptide sequence has been removed (1-105)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (C terminal on backbone)
    • TamA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspHI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pTAMACHis6 expression vector was obtained by replacing the PelB signal peptide of pEPELBCHIS (courtesy of Huanting Liu, University St Andrews) with the TamA signal peptide

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAMACHis6_PiuD was a gift from James Naismith (Addgene plasmid # 128953 ; http://n2t.net/addgene:128953 ; RRID:Addgene_128953)

  • For your References section:

    TonB-Dependent Receptor Repertoire of Pseudomonas aeruginosa for Uptake of Siderophore-Drug Conjugates. Luscher A, Moynie L, Auguste PS, Bumann D, Mazza L, Pletzer D, Naismith JH, Kohler T. Antimicrob Agents Chemother. 2018 May 25;62(6). pii: AAC.00097-18. doi: 10.1128/AAC.00097-18. Print 2018 Jun. 10.1128/AAC.00097-18 PubMed 29555629
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