pTAMAHISTEV_BauA
(Plasmid #128952)

• Purpose: expresses BauA in E.coli
• Depositing Lab: James Naismith
• Publication: Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 10.7554/eLife.42270.

Backbone

• Vector backbone: pTAMAHISTEV
• Backbone size w/o insert (bp): 5566
• Total vector size (bp): 7669
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: protein expression in C43(DE3) strains
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ferric acinetobactin receptor
• Alt name: BauA
• Species: Acinetobacter baumannii ATCC 19606
• Insert Size (bp): 2112
• Mutation: Signal peptide sequence has been removed (1-66)
• GenBank ID: BAC87903.1
• Promoter: T7
• Tags / Fusion Proteins:
  • TEV protease cleavable 7xHis (N terminal on backbone)
  • TamA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pTAMAHISTEV vector has been obtained by replacing pelB signal peptide of pEPELBHISTEV (courtesy of Dr. Huanting Liu, University St Andrews) by tamA signal peptide