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pTAMAHISTEV_PfeAR480A-Q482A
(Plasmid #128946)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTAMAHISTEV
  • Backbone size w/o insert (bp) 5566
  • Total vector size (bp) 7719
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    protein expression in C43(DE3) strains
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ferric enterobactin receptor
  • Alt name
    PfeA
  • Alt name
    PA2688
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    2166
  • Mutation
    Signal peptide sequence has been removed (1-75), mutations Arginine 480 to Alanine and Glutamine 482 to Alanine
  • GenBank ID
    882784
  • Promoter T7
  • Tags / Fusion Proteins
    • TEV protease cleavable 7xHis (N terminal on backbone)
    • TamA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pTAMAHISTEV vector has been obtained by replacing pelB signal peptide of pEPELBHISTEV (courtesy of Dr. Huanting Liu, University St Andrews) by tamA signal peptide

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAMAHISTEV_PfeAR480A-Q482A was a gift from James Naismith (Addgene plasmid # 128946 ; http://n2t.net/addgene:128946 ; RRID:Addgene_128946)

  • For your References section:

    The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model. Moynie L, Milenkovic S, Mislin GLA, Gasser V, Malloci G, Baco E, McCaughan RP, Page MGP, Schalk IJ, Ceccarelli M, Naismith JH. Nat Commun. 2019 Aug 14;10(1):3673. doi: 10.1038/s41467-019-11508-y. 10.1038/s41467-019-11508-y PubMed 31413254
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