pTAMAHISTEV_PfeAR480A
(Plasmid
#128944)
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Purposeexpresses R480A mutant of PfeA in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTAMAHISTEV
- Backbone size w/o insert (bp) 5566
- Total vector size (bp) 7719
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsprotein expression in C43(DE3) strains
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameferric enterobactin receptor
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Alt namePfeA
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Alt namePA2688
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SpeciesPseudomonas aeruginosa PAO1
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Insert Size (bp)2166
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MutationSignal peptide sequence has been removed (1-75), mutations Arginine 480 to Alanine
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GenBank ID882784
- Promoter T7
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Tags
/ Fusion Proteins
- TEV protease cleavable 7xHis (N terminal on backbone)
- TamA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pTAMAHISTEV vector has been obtained by replacing pelB signal peptide of pEPELBHISTEV (courtesy of Dr. Huanting Liu, University St Andrews) by tamA signal peptide
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTAMAHISTEV_PfeAR480A was a gift from James Naismith (Addgene plasmid # 128944 ; http://n2t.net/addgene:128944 ; RRID:Addgene_128944) -
For your References section:
The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model. Moynie L, Milenkovic S, Mislin GLA, Gasser V, Malloci G, Baco E, McCaughan RP, Page MGP, Schalk IJ, Ceccarelli M, Naismith JH. Nat Commun. 2019 Aug 14;10(1):3673. doi: 10.1038/s41467-019-11508-y. 10.1038/s41467-019-11508-y PubMed 31413254