pEcRNAP6
(Plasmid #128940)

• Purpose: pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
• Depositing Lab: Seth Darst
• Publication: Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi: 10.1002/pro.622. Epub 2011 Apr 26.

Backbone

• Vector backbone: pET21a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: RpoA
• Alt name: RNA polymerase subunit alpha
• Species: E. coli
• GenBank ID: 947794
• Entrez Gene: rpoA (a.k.a. b3295, ECK3282, pez, phs, sez)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: NcoI (unknown if destroyed)
• 5′ sequencing primer: T7 Promotor

Gene/Insert 2

• Gene/Insert name: RpoB
• Species: Escherichia coli
• GenBank ID: 948488

Gene/Insert 3

• Gene/Insert name: RpoC-ppx(His)10
• Species: Escherichia coli
• Mutation: PreScission protease site (GE Healthcare; LEVLFQGP) is inserted between the C-terminus of RpoC and the (His)10-tag
• GenBank ID: 948487
• Tag / Fusion Protein:
  • His (C terminal on insert)

Gene/Insert 4

• Gene/Insert name: RpoZ
• Species: Escherichia coli
• GenBank ID: 948160

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry