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Addgene

pEcRNAP5
(Plasmid #128939)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128939 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    RpoAppx234-241-(His)10
  • Species
    Escherichia coli
  • Insert Size (bp)
    1025
  • Mutation
    Residues 234-241 are substituted for a PreScission protease site (GE Healthcare: LEVLFQGP).
  • GenBank ID
    947794
  • Entrez Gene
    rpoA (a.k.a. b3295, ECK3282, pez, phs, sez)
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site NcoI (unknown if destroyed)
  • 5′ sequencing primer T7 Promotor
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RpoB
  • Species
    Escherichia coli
  • GenBank ID
    948488

Gene/Insert 3

  • Gene/Insert name
    RpoC
  • Species
    Escherichia coli
  • GenBank ID
    948487

Gene/Insert 4

  • Gene/Insert name
    RpoZ
  • Species
    Escherichia coli
  • GenBank ID
    948160

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEcRNAP5 was a gift from Seth Darst (Addgene plasmid # 128939 ; http://n2t.net/addgene:128939 ; RRID:Addgene_128939)
  • For your References section:

    A novel method for the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking the alpha C-terminal domain. Twist KA, Husnain SI, Franke JD, Jain D, Campbell EA, Nickels BE, Thomas MS, Darst SA, Westblade LF. Protein Sci. 2011 Jun;20(6):986-95. doi: 10.1002/pro.622. Epub 2011 Apr 26. 10.1002/pro.622 PubMed 21416542