JB54
(Plasmid
#128925)
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Purposemammalian expression, proximity labelling, mitochondrial relocalisation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 128925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecDNA3.28
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRac1TN-BirA*-HA-MAO
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SpeciesH. sapiens (human)
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Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/553206v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JB54 was a gift from Sean Munro (Addgene plasmid # 128925 ; http://n2t.net/addgene:128925 ; RRID:Addgene_128925) -
For your References section:
In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Gillingham AK, Bertram J, Begum F, Munro S. Elife. 2019 Jul 11;8. pii: 45916. doi: 10.7554/eLife.45916. 10.7554/eLife.45916 PubMed 31294692