pMV16
(Plasmid
#128894)
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PurposePosition 6 module- LEU2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 128894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57BsaI
- Backbone size w/o insert (bp) 2724
- Total vector size (bp) 4967
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Modifications to backboneInsertion of AGCA-LEU2-CCAT by MEGAWHOP
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Vector typeYeast Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLEU2
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2243
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/630277v1 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMV16 was a gift from Gilles Charvin (Addgene plasmid # 128894 ; http://n2t.net/addgene:128894 ; RRID:Addgene_128894) -
For your References section:
COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast. Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G. PLoS One. 2019 Aug 28;14(8):e0220694. doi: 10.1371/journal.pone.0220694. eCollection 2019. 10.1371/journal.pone.0220694 PubMed 31461461