pEGFPC1-Cep83
(Plasmid
#128874)
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PurposeFor mammalian expression of mouse Cep83
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128874 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCep83
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Alt nameCcdc41
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SpeciesM. musculus (mouse)
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Entrez GeneCcdc41 (a.k.a. 2600001G24Rik, 4921537D05Rik, 5730513H21Rik)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPC1-Cep83 was a gift from Aimin Liu (Addgene plasmid # 128874 ; http://n2t.net/addgene:128874 ; RRID:Addgene_128874) -
For your References section:
C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals. Ye X, Zeng H, Ning G, Reiter JF, Liu A. Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2164-9. doi: 10.1073/pnas.1318737111. Epub 2014 Jan 27. 10.1073/pnas.1318737111 PubMed 24469809