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Addgene

pEGFPC1-Spop
(Plasmid #128872)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128872 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spop
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Spop (a.k.a. Pcif1, TEF2)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-Spop was a gift from Aimin Liu (Addgene plasmid # 128872 ; http://n2t.net/addgene:128872 ; RRID:Addgene_128872)
  • For your References section:

    Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling. Cai H, Liu A. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14751-14756. doi: 10.1073/pnas.1612520114. Epub 2016 Dec 5. 10.1073/pnas.1612520114 PubMed 27930311