pEGFPC1-Spop
(Plasmid
#128872)
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PurposeFor mammalian expression of mouse Spop
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128872 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpop
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SpeciesM. musculus (mouse)
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Entrez GeneSpop (a.k.a. Pcif1, TEF2)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPC1-Spop was a gift from Aimin Liu (Addgene plasmid # 128872 ; http://n2t.net/addgene:128872 ; RRID:Addgene_128872) -
For your References section:
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling. Cai H, Liu A. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14751-14756. doi: 10.1073/pnas.1612520114. Epub 2016 Dec 5. 10.1073/pnas.1612520114 PubMed 27930311