pEGFPC3-C2cd3
(Plasmid
#128871)
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PurposeFor mammalian expression of mouse C2cd3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 128871 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameC2cd3
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SpeciesM. musculus (mouse)
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Entrez GeneC2cd3 (a.k.a. AU020772)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPC3-C2cd3 was a gift from Aimin Liu (Addgene plasmid # 128871 ; http://n2t.net/addgene:128871 ; RRID:Addgene_128871) -
For your References section:
C2cd3 is required for cilia formation and Hedgehog signaling in mouse. Hoover AN, Wynkoop A, Zeng H, Jia J, Niswander LA, Liu A. Development. 2008 Dec;135(24):4049-58. doi: 10.1242/dev.029835. Epub 2008 Nov 12. 10.1242/dev.029835 PubMed 19004860