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Addgene

pcDNA-FLAG-Bbs4
(Plasmid #128868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bbs4
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Bbs4 (a.k.a. D9Ertd464e)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-FLAG-Bbs4 was a gift from Aimin Liu (Addgene plasmid # 128868 ; http://n2t.net/addgene:128868 ; RRID:Addgene_128868)

  • For your References section:

    C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals. Ye X, Zeng H, Ning G, Reiter JF, Liu A. Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2164-9. doi: 10.1073/pnas.1318737111. Epub 2014 Jan 27. 10.1073/pnas.1318737111 PubMed 24469809
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