pD441_WT_hi
(Plasmid
#128857)
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PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepD441
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Backbone manufacturerATUM
- Backbone size w/o insert (bp) 4000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlagellin
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SpeciesE. coli
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Insert Size (bp)1497
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pD441_WT_hi was a gift from Mark Pallen (Addgene plasmid # 128857 ; http://n2t.net/addgene:128857 ; RRID:Addgene_128857) -
For your References section:
Restoration of wild-type motility to flagellin-knockout Escherichia coli by varying promoter, copy number and induction strength in plasmid-based expression of flagellin. Thomson NM, Pallen MJ. Curr Res Biotechnol. 2020 Nov;2:45-52. doi: 10.1016/j.crbiot.2020.03.001. 10.1016/j.crbiot.2020.03.001 PubMed 33381753
