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pJ211_rha_WT_hi
(Plasmid #128853)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128853 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ211
  • Backbone manufacturer
    ATUM
  • Backbone size w/o insert (bp) 2500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flagellin
  • Species
    E. coli
  • Insert Size (bp)
    1497

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ211_rha_WT_hi was a gift from Mark Pallen (Addgene plasmid # 128853 ; http://n2t.net/addgene:128853 ; RRID:Addgene_128853)

  • For your References section:

    Restoration of wild-type motility to flagellin-knockout Escherichia coli by varying promoter, copy number and induction strength in plasmid-based expression of flagellin. Thomson NM, Pallen MJ. Curr Res Biotechnol. 2020 Nov;2:45-52. doi: 10.1016/j.crbiot.2020.03.001. 10.1016/j.crbiot.2020.03.001 PubMed 33381753
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