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Addgene

pR-spondin 1
(Plasmid #128844)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128844 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-DUET
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositor uses bacterial strain Shuffle for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    R-spondin 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1587
  • Entrez Gene
    RSPO1 (a.k.a. CRISTIN3, RSPO)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer DuetDOWN1 primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Augen Pioszak, at the Board of Regents of the University of Oklahoma, Health Sciences Center, provided the original plasmid that was modified, as detailed, in PMID-30996238

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pR-spondin 1 was a gift from Marc de la Roche (Addgene plasmid # 128844 ; http://n2t.net/addgene:128844 ; RRID:Addgene_128844)

  • For your References section:

    Organoid culture media formulated with growth factors of defined cellular activity. Urbischek M, Rannikmae H, Foets T, Ravn K, Hyvonen M, de la Roche M. Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.1038/s41598-019-42604-0. 10.1038/s41598-019-42604-0 PubMed 30996238
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