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Addgene

Tg(mpeg1:EGFP-Mmu.Rpl10a)
(Plasmid #128840)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128840 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Tol2:mpeg-GFP
  • Total vector size (bp) 6727
  • Vector type
    zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP-L10a
  • Insert Size (bp)
    1400

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site kpnI (unknown if destroyed)
  • 3′ cloning site xbaI (unknown if destroyed)
  • 5′ sequencing primer gtgaaagtacaa
  • 3′ sequencing primer ccagaggtgtaa
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tg(mpeg1:EGFP-Mmu.Rpl10a) was a gift from Anna Huttenlocher (Addgene plasmid # 128840 ; http://n2t.net/addgene:128840 ; RRID:Addgene_128840)
  • For your References section:

    Heat shock modulates neutrophil motility in zebrafish. Lam PY, Harvie EA, Huttenlocher A. PLoS One. 2013 Dec 19;8(12):e84436. doi: 10.1371/journal.pone.0084436. eCollection 2013. 10.1371/journal.pone.0084436 PubMed 24367659