Tg(lyz:EGFP-Mmu.Rpl10a)
(Plasmid
#128838)
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PurposeRNA isolation from neutrophils via TRAP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneTol2:mpeg-GFP
- Total vector size (bp) 7328
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Vector typezebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP-L10a
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Insert Size (bp)1400
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site kpnI (unknown if destroyed)
- 3′ cloning site xbaI (unknown if destroyed)
- 5′ sequencing primer gtgaaagtacaa
- 3′ sequencing primer ccagaggtgtaa (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tg(lyz:EGFP-Mmu.Rpl10a) was a gift from Anna Huttenlocher (Addgene plasmid # 128838 ; http://n2t.net/addgene:128838 ; RRID:Addgene_128838) -
For your References section:
Heat shock modulates neutrophil motility in zebrafish. Lam PY, Harvie EA, Huttenlocher A. PLoS One. 2013 Dec 19;8(12):e84436. doi: 10.1371/journal.pone.0084436. eCollection 2013. 10.1371/journal.pone.0084436 PubMed 24367659
Map uploaded by the depositor.
