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Addgene

pACYCDuet-1_Ec_rpoZ
(Plasmid #128837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYCDuet-1
  • Backbone size w/o insert (bp) 3939
  • Total vector size (bp) 4215
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rpoZ
  • Species
    E. coli
  • Insert Size (bp)
    276
  • Entrez Gene
    rpoZ (a.k.a. b3649, ECK3639, spoS)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 Promotor
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYCDuet-1_Ec_rpoZ was a gift from Seth Darst (Addgene plasmid # 128837 ; http://n2t.net/addgene:128837 ; RRID:Addgene_128837)

  • For your References section:

    X-ray Crystal Structure of Escherichia coli RNA Polymerase sigma70 Holoenzyme. Murakami KS. J Biol Chem. 2013 Mar 29;288(13):9126-34. doi: 10.1074/jbc.M112.430900. Epub 2013 Feb 6. 10.1074/jbc.M112.430900 PubMed 23389035
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