pFSW Doc2alpha Halotag
(Plasmid
#128817)
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PurposeTo make virus expressing Doc2alpha Halotag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
- Backbone size w/o insert (bp) 10657
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDoc2alpha
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Alt nameDouble C2 protein alpha
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1209
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Entrez GeneDoc2a
- Promoter hSyn (human synapsin I promoter)
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Tag
/ Fusion Protein
- HaloTag-IRES-EGFP (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFSW Doc2alpha Halotag was a gift from Edwin Chapman (Addgene plasmid # 128817 ; http://n2t.net/addgene:128817 ; RRID:Addgene_128817) -
For your References section:
Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors and Sources to Regulate Spontaneous Release. Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER. Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 10.1016/j.neuron.2018.04.022. Epub 2018 May 10. 10.1016/j.neuron.2018.04.022 PubMed 29754754