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Addgene

pFSW Doc2alpha D291N mutant
(Plasmid #128813)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128813 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone size w/o insert (bp) 9697
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Doc2alpha
  • Alt name
    Double C2 protein alpha
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1209
  • Mutation
    D291N
  • Entrez Gene
    Doc2a
  • Promoter hSyn (human synapsin I promoter)
  • Tag / Fusion Protein
    • IRES-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFSW Doc2alpha D291N mutant was a gift from Edwin Chapman (Addgene plasmid # 128813 ; http://n2t.net/addgene:128813 ; RRID:Addgene_128813)

  • For your References section:

    Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors and Sources to Regulate Spontaneous Release. Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER. Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 10.1016/j.neuron.2018.04.022. Epub 2018 May 10. 10.1016/j.neuron.2018.04.022 PubMed 29754754
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