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Purposeoverexpression of DDX21 in human cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE-EF1a-IRES-ZsGreen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDDX21 WT
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SpeciesH. sapiens (human)
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Insert Size (bp)2352
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Tag
/ Fusion Protein
- flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p23-DDX21 WT was a gift from Ling-Ling Chen (Addgene plasmid # 128803 ; http://n2t.net/addgene:128803 ; RRID:Addgene_128803) -
For your References section:
SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Xing YH, Yao RW, Zhang Y, Guo CJ, Jiang S, Xu G, Dong R, Yang L, Chen LL. Cell. 2017 May 4;169(4):664-678.e16. doi: 10.1016/j.cell.2017.04.011. 10.1016/j.cell.2017.04.011 PubMed 28475895