pLZts
(Plasmid
#128799)
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Purpose(Empty Backbone) Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128799 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLZ12-Spec
- Backbone size (bp) 3733
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Modifications to backboneReplaced wild type RepA gene with temperature-sensitive RepA from pJRS233
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Vector typeBacterial mutagenesis
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsTemperature-sensitive. Routine growth at 28-30C
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLZts was a gift from Timothy Barnett (Addgene plasmid # 128799 ; http://n2t.net/addgene:128799 ; RRID:Addgene_128799) -
For your References section:
Genetic Manipulation of Group A Streptococcus-Gene Deletion by Allelic Replacement. Barnett TC, Daw JN, Walker MJ, Brouwer S. Methods Mol Biol. 2020;2136:59-69. doi: 10.1007/978-1-0716-0467-0_5. 10.1007/978-1-0716-0467-0_5 PubMed 32430813
