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Addgene

pLG327
(Plasmid #128797)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLG166
  • Backbone size w/o insert (bp) 7675
  • Total vector size (bp) 8386
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gfpmut2
  • Alt name
    has a different xylose promoter without the CRE site
  • Species
    Synthetic
  • Entrez Gene
    NEWENTRY
  • Promoter Pxyl(s)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TGGCATACGCGTAATAAAGTGATGAATC
  • 3′ sequencing primer GCACGTGTCTTGTAGTTCCCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLG327 was a gift from Christopher Voigt (Addgene plasmid # 128797 ; http://n2t.net/addgene:128797 ; RRID:Addgene_128797)

  • For your References section:

    Resilient living materials built by printing bacterial spores. Gonzalez LM, Mukhitov N, Voigt CA. Nat Chem Biol. 2019 Dec 2. pii: 10.1038/s41589-019-0412-5. doi: 10.1038/s41589-019-0412-5. 10.1038/s41589-019-0412-5 PubMed 31792444
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