Skip to main content
Addgene

pCambia2300 - ProRUBY:RUBY-Citrine
(Plasmid #128796)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128796 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCambia2300
  • Backbone manufacturer
    Cambia
  • Backbone size w/o insert (bp) 8711
  • Total vector size (bp) 15884
  • Vector type
    Plant Expression
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RUBY PARTICLES IN MUCILAGE
  • Alt name
    RUBY
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    6128
  • GenBank ID
    NM_101845.3
  • Entrez Gene
    AT1G19900 (a.k.a. AT1G19900, F6F9.4, F6F9_4)
  • Promoter ProRUBY
  • Tag / Fusion Protein
    • Citrine (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer caggaaacagctatgac
  • 3′ sequencing primer gcatcgagctgaagggcat
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCambia2300 - ProRUBY:RUBY-Citrine was a gift from George Haughn (Addgene plasmid # 128796 ; http://n2t.net/addgene:128796 ; RRID:Addgene_128796)

  • For your References section:

    RUBY, a Putative Galactose Oxidase, Influences Pectin Properties and Promotes Cell-To-Cell Adhesion in the Seed Coat Epidermis of Arabidopsis. Sola K, Gilchrist EJ, Ropartz D, Wang L, Feussner I, Mansfield SD, Ralet MC, Haughn GW. Plant Cell. 2019 Apr;31(4):809-831. doi: 10.1105/tpc.18.00954. Epub 2019 Mar 8. 10.1105/tpc.18.00954 PubMed 30852555
Related items:
Commonly requested with: