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Addgene

pET29b-pld
(Plasmid #128795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5229
  • Total vector size (bp) 6660
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phospholipase D
  • Alt name
    PLD, pld
  • Species
    E. coli
  • Insert Size (bp)
    1431
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTTTAACTTTAAGAAGGAGATATAATGAATGATTTGCCTCGGCTGGCGAGCGCGGTGCTGC
  • 3′ sequencing primer CAAACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAACACCACCACCACCACCACTGAGATC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-pld was a gift from Emily Balskus (Addgene plasmid # 128795 ; http://n2t.net/addgene:128795 ; RRID:Addgene_128795)

  • For your References section:

    Gut bacterial phospholipase Ds support disease-associated metabolism by generating choline. Chittim CL, Martinez Del Campo A, Balskus EP. Nat Microbiol. 2019 Jan;4(1):155-163. doi: 10.1038/s41564-018-0294-4. Epub 2018 Nov 19. 10.1038/s41564-018-0294-4 PubMed 30455469
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