pGST-TEV-yVms1(1-417,delta(38-69))
(Plasmid #128739)

• Purpose: Bacterial overexpression and crystallization
• Depositing Lab: Chris Hill
• Publication: Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.

Backbone

• Vector backbone: pGST parallel
• Backbone size w/o insert (bp): 4866
• Total vector size (bp): 6127
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1)
• Alt name: YDR049W
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1161
• Mutation: residues 1-417 with deletion of 38-69
• Entrez Gene: VMS1 (a.k.a. YDR049W)
• Promoter: T7
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: ctttgcagggctggc

Resource Information

• Supplemental Documents:
  • pJR13811_genbank.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains an A147V mutation in Vms1. This mutation is not known to affect plasmid function.