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Addgene

pGST-TEV-yVms1(1-417,delta(38-69))
(Plasmid #128739)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128739 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGST parallel
  • Backbone size w/o insert (bp) 4866
  • Total vector size (bp) 6127
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1)
  • Alt name
    YDR049W
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1161
  • Mutation
    residues 1-417 with deletion of 38-69
  • Entrez Gene
    VMS1 (a.k.a. YDR049W)
  • Promoter T7
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer ctttgcagggctggc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains an A147V mutation in Vms1. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST-TEV-yVms1(1-417,delta(38-69)) was a gift from Chris Hill (Addgene plasmid # 128739 ; http://n2t.net/addgene:128739 ; RRID:Addgene_128739)
  • For your References section:

    Sterol Oxidation Mediates Stress-Responsive Vms1 Translocation to Mitochondria. Nielson JR, Fredrickson EK, Waller TC, Rendon OZ, Schubert HL, Lin Z, Hill CP, Rutter J. Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022. 10.1016/j.molcel.2017.10.022 PubMed 29149595