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MBP-TEV-Gly-RadA C46
(Plasmid #128736)

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Item Catalog # Description Quantity Price (USD)
Plasmid 128736 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pMAL-c5X
  • Backbone size w/o insert (bp) 5677
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RadA
  • Species
    Pyrococcus horikoshii
  • Promoter T7
  • Tag / Fusion Protein
    • Hisx6-MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-TEV-Gly-RadA C46 was a gift from Sharon Rozovsky (Addgene plasmid # 128736 ; http://n2t.net/addgene:128736 ; RRID:Addgene_128736)

  • For your References section:

    Efficient generation of hydrazides in proteins by RadA split intein. Liu J, Ekanayake O, Santoleri D, Walker K, Rozovsky S. Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160. 10.1002/cbic.201900160 PubMed 31265209
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