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Addgene

pCPP6450
(Plasmid #128731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/SD/D-TOPO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HopAD1
  • Species
    Pseudomonas syringae

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also reference: PMID 29742421.

Addgene QC identified an unexpected IS element present in the plasmid backbone. The depositing lab does not believe this to be of functional concern but requesting scientists should confirm the functionality of the construct prior to experimentation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCPP6450 was a gift from Alan Collmer (Addgene plasmid # 128731 ; http://n2t.net/addgene:128731 ; RRID:Addgene_128731)

  • For your References section:

    Pseudomonas syringae pv. tomato DC3000 Type III Secretion Effector Polymutants Reveal an Interplay between HopAD1 and AvrPtoB. Wei HL, Chakravarthy S, Mathieu J, Helmann TC, Stodghill P, Swingle B, Martin GB, Collmer A. Cell Host Microbe. 2015 Jun 10;17(6):752-62. doi: 10.1016/j.chom.2015.05.007. 10.1016/j.chom.2015.05.007 PubMed 26067603
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