pCPP5233
(Plasmid
#128715)
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PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/SD/D-TOPO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshcM-hopM1
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SpeciesPseudomonas syringae
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also reference: PMID 29742421.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCPP5233 was a gift from Alan Collmer (Addgene plasmid # 128715 ; http://n2t.net/addgene:128715 ; RRID:Addgene_128715) -
For your References section:
Deletions in the repertoire of Pseudomonas syringae pv. tomato DC3000 type III secretion effector genes reveal functional overlap among effectors. Kvitko BH, Park DH, Velasquez AC, Wei CF, Russell AB, Martin GB, Schneider DJ, Collmer A. PLoS Pathog. 2009 Apr;5(4):e1000388. doi: 10.1371/journal.ppat.1000388. Epub 2009 Apr 17. 10.1371/journal.ppat.1000388 PubMed 19381254