pDM304_DPAKa(GBD)-YFP
(Plasmid
#128699)
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Purposeexpression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128699 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDM304
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Backbone manufacturerVeltman et al (2009)
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Vector typeDictyostelium discoideum expression vector
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGBD domain (aa 731-890) of DPAKa
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Alt namepakA
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Alt namedpak1
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SpeciesDictyostelium discoideum
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Mutationnone
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS results verified the insert of this plasmid but cannot confirm the entire backbone sequence. We recommend doing additional diagnostic tests before use. Depositor confirms plasmid functions as described in their publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDM304_DPAKa(GBD)-YFP was a gift from Igor Weber (Addgene plasmid # 128699 ; http://n2t.net/addgene:128699 ; RRID:Addgene_128699) -
For your References section:
Quantitative imaging of Rac1 activity in Dictyostelium cells with a fluorescently labelled GTPase-binding domain from DPAKa kinase. Marinovic M, Sostar M, Filic V, Antolovic V, Weber I. Histochem Cell Biol. 2016 Sep;146(3):267-79. doi: 10.1007/s00418-016-1440-9. Epub 2016 Apr 28. 10.1007/s00418-016-1440-9 PubMed 27126594