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pDM304_YFP-IqgC_mRFP-Raf1(RBD)
(Plasmid #128698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDM304
  • Backbone manufacturer
    Veltman et al (2009)
  • Vector type
    Dictyostelium discoideum expression vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    iqgC (full length)
  • Species
    Dictyostelium discoideum
  • Mutation
    none
  • Tag / Fusion Protein
    • YFP (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Raf1 kinase (aa 50-132)
  • Species
    M. musculus (mouse)
  • Mutation
    none
  • Tag / Fusion Protein
    • mRFP (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results verified the insert of this plasmid but cannot confirm the entire backbone sequence. We recommend doing additional diagnostic tests before use. Depositor confirms plasmid functions as described in their publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDM304_YFP-IqgC_mRFP-Raf1(RBD) was a gift from Igor Weber (Addgene plasmid # 128698 ; http://n2t.net/addgene:128698 ; RRID:Addgene_128698)

  • For your References section:

    IQGAP-related protein IqgC suppresses Ras signaling during large-scale endocytosis. Marinovic M, Mijanovic L, Sostar M, Vizovisek M, Junemann A, Fonovic M, Turk B, Weber I, Faix J, Filic V. Proc Natl Acad Sci U S A. 2019 Jan 22;116(4):1289-1298. doi: 10.1073/pnas.1810268116. Epub 2019 Jan 8. 10.1073/pnas.1810268116 PubMed 30622175
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