pT3TS-TAL-apobb.1-PR2-LA
(Plasmid #128695)

• Purpose: Left arm of TALEN pair 2 for cleavage of apobb.1 STOP locus
• Depositing Lab: Steven Farber
• Publication: Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi: 10.1038/s41467-019-11259-w.

Backbone

• Vector backbone: pT3TS-delta-TAL
• Backbone manufacturer: Ekker Lab
• Backbone size w/o insert (bp): 4651
• Total vector size (bp): 5940
• Modifications to backbone: Golden gate assembly reaction
• Vector type: TALEN

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TALEN
• Insert Size (bp): 2203

Resource Information

• Supplemental Documents:
  • pT3TS-TAL-PR2-LA.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains a lot of repetitive regions and thus next generation sequencing had difficulty assembling a full plasmid sequence. Due to this Addgene's full plasmid sequence contains several ambiguous bases and a few mismatches in non-essential regions compared to depositor sequence. Depositor confirms plasmid is functional!