pDestTol2pA2-LA-nanoLuc-RA
(Plasmid
#128692)
-
PurposeDonor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locus
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 128692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDestTol2pA2
-
Backbone manufacturerUtah Genetics
- Backbone size w/o insert (bp) 5883
- Total vector size (bp) 6086
-
Modifications to backboneMultisite-Gateway assembly reaction (LR)
-
Vector typeMultisite-Gateway assembly
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsSlow growth and low yield from DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameapobb.1 NanoLuc
-
SpeciesD. rerio (zebrafish)
-
Insert Size (bp)1868
-
GenBank IDENSDARG00000022767
-
Tag
/ Fusion Protein
- NanoLuc
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDestTol2pA2-LA-nanoLuc-RA was a gift from Steven Farber (Addgene plasmid # 128692 ; http://n2t.net/addgene:128692 ; RRID:Addgene_128692) -
For your References section:
The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins. Thierer JH, Ekker SC, Farber SA. Nat Commun. 2019 Jul 31;10(1):3426. doi: 10.1038/s41467-019-11259-w. 10.1038/s41467-019-11259-w PubMed 31366908