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Addgene

pDestTol2pA2-LA-nanoLuc-RA
(Plasmid #128692)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128692 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDestTol2pA2
  • Backbone manufacturer
    Utah Genetics
  • Backbone size w/o insert (bp) 5883
  • Total vector size (bp) 6086
  • Modifications to backbone
    Multisite-Gateway assembly reaction (LR)
  • Vector type
    Multisite-Gateway assembly

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Slow growth and low yield from DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    apobb.1 NanoLuc
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1868
  • GenBank ID
    ENSDARG00000022767
  • Tag / Fusion Protein
    • NanoLuc

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2pA2-LA-nanoLuc-RA was a gift from Steven Farber (Addgene plasmid # 128692 ; http://n2t.net/addgene:128692 ; RRID:Addgene_128692)
  • For your References section:

    The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins. Thierer JH, Ekker SC, Farber SA. Nat Commun. 2019 Jul 31;10(1):3426. doi: 10.1038/s41467-019-11259-w. 10.1038/s41467-019-11259-w PubMed 31366908