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Addgene

His-TEV-GB1 Val54Met
(Plasmid #128691)

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Item Catalog # Description Quantity Price (USD)
Plasmid 128691 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pJexpress414
  • Backbone size w/o insert (bp) 3973
  • Total vector size (bp) 4226
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spg
  • Species
    Streptococcus sp. group G
  • Insert Size (bp)
    235
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA 2.0

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-TEV-GB1 Val54Met was a gift from Sharon Rozovsky (Addgene plasmid # 128691 ; http://n2t.net/addgene:128691 ; RRID:Addgene_128691)

  • For your References section:

    (77)Se NMR Probes the Protein Environment of Selenomethionine. Chen Q, Xu S, Lu X, Boeri MV, Pepelyayeva Y, Diaz EL, Soni SD, Allaire M, Forstner MB, Bahnson BJ, Rozovsky S. J Phys Chem B. 2020 Jan 30;124(4):601-616. doi: 10.1021/acs.jpcb.9b07466. Epub 2020 Jan 7. 10.1021/acs.jpcb.9b07466 PubMed 31846581
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