Skip to main content
Addgene

pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro
(Plasmid #128666)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCT--CMV-IRES-puro
  • Backbone size w/o insert (bp) 8048
  • Total vector size (bp) 9186
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PolB
  • Alt name
    DNA polymerase beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1038
  • Mutation
    L301R/V303R/V306R
  • GenBank ID
    NM_002690
  • Entrez Gene
    POLB
  • Promoter CMV
  • Tag / Fusion Protein
    • copGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CAA TTC TGC CGT GGA CGG CAC
  • 3′ sequencing primer GCG ATG TGC GCT CTG CCC ACT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro was a gift from Robert Sobol (Addgene plasmid # 128666 ; http://n2t.net/addgene:128666 ; RRID:Addgene_128666)

  • For your References section:

    HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase beta. Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW. Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513. 10.1038/ncomms6513 PubMed 25423885
Related items: