pLVX-EGFP-IRES-puro
(Plasmid #128652)

• Purpose: mammalian expression of EGFP with puromycin selection
• Depositing Lab: Robert Sobol
• Publication: Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.

Backbone

• Vector backbone: pLVX-GWE-IRES-puro
• Backbone size w/o insert (bp): 8247
• Total vector size (bp): 8958
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP
• Alt name: N/A
• Insert Size (bp): 720
• Mutation: Wild type
• GenBank ID: NC_025025.1
• Promoter: CMV
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 forward for pENTR vector
• 3′ sequencing primer: M13 reverse for pENTR vector

Resource Information

• Supplemental Documents:
  • 727-pLVX-EGFP-IRES-Puro.ape
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry