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Addgene

pIIS18-BufAI-2
(Plasmid #128651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    self-made
  • Backbone size (bp) 2562
  • Vector type
    Unspecified
  • Selectable markers
    lacZ alpha

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIIS18-BufAI-2 was a gift from Tohru Suzuki (Addgene plasmid # 128651 ; http://n2t.net/addgene:128651 ; RRID:Addgene_128651)

  • For your References section:

    A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes. Altaib H, Ozaki Y, Kozakai T, Badr Y, Nomura I, Suzuki T. Microbiol Resour Announc. 2019 Oct 10;8(41). pii: 8/41/e00323-19. doi: 10.1128/MRA.00323-19. 10.1128/MRA.00323-19 PubMed 31601653
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