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pARS3_MUbCAS9_GW
(Plasmid #128646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pARS3
  • Backbone manufacturer
    Thilmony,R.
  • Backbone size (bp) 7000
  • Modifications to backbone
    The T-DNA portion of the vector incorporates a transformation booster sequence (TBS), a plant-codon-optimized hemagglutinin (HA)- tagged nuclear-localized Cas9 gene (Cas9-HA-N7), driven by the Zea mays UBQ10 promoter and containing a ribosomal-binding site (RBS), and OCS terminator. Gateway cassette from pEarlyGate103 was added to allow Gateway cloning of gRNAs.
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • HA

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    It is growing slower due to big size.
  • Copy number
    High Copy

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    All features but Gateway cassette was cloned into pARS3m vector in Zachary Nimchuk lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pARS3_MUbCAS9_GW was a gift from G. Eric Schaller (Addgene plasmid # 128646 ; http://n2t.net/addgene:128646 ; RRID:Addgene_128646)

  • For your References section:

    Type-B response regulators of rice play key roles in growth, development, and cytokinin signaling. Worthen JM, Yamburenko MV, Lim J, Nimchuk ZL, Kieber JJ, Schaller GE. Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870. 10.1242/dev.174870 PubMed 31160418
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