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pET28a-INTS3-C
(Plasmid #128642)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128642 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6889
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INTS3 C-termini
  • Alt name
    Integrator Complex Subunit 3 (INTS3)
  • Alt name
    SOSS-A
  • Alt name
    C1orf60
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1587
  • Mutation
    INTS3 C-termini (514–1042 aa)
  • GenBank ID
    NM_023015.5
  • Entrez Gene
    INTS3 (a.k.a. C1orf193, C1orf60, INT3, SOSS-A, SOSSA)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag on N and C-terminal

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-INTS3-C was a gift from Yuliang Wu (Addgene plasmid # 128642 ; http://n2t.net/addgene:128642 ; RRID:Addgene_128642)

  • For your References section:

    Biochemical characterization of INTS3 and C9ORF80, two subunits of hNABP1/2 heterotrimeric complex in nucleic acid binding. Vidhyasagar V, He Y, Guo M, Talwar T, Singh RS, Yadav M, Katselis G, Vizeacoumar FJ, Lukong KE, Wu Y. Biochem J. 2018 Jan 2;475(1):45-60. doi: 10.1042/BCJ20170351. 10.1042/BCJ20170351 PubMed 29150435
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