smHA multi-frame tag
(Plasmid
#128611)
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Purposea spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128611 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHAGE-UbiC
- Total vector size (bp) 11231
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesmHA MF tag
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
smHA multi-frame tag was a gift from Tim Stasevich (Addgene plasmid # 128611 ; http://n2t.net/addgene:128611 ; RRID:Addgene_128611) -
For your References section:
Live-Cell Single RNA Imaging Reveals Bursts of Translational Frameshifting. Lyon K, Aguilera LU, Morisaki T, Munsky B, Stasevich TJ. Mol Cell. 2019 Jul 11;75(1):172-183.e9. doi: 10.1016/j.molcel.2019.05.002. Epub 2019 Jun 6. 10.1016/j.molcel.2019.05.002 PubMed 31178355
