12xMoonTag-12xSunTag-kif18b-24xPP7
(Plasmid #128606)

• Purpose: Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
• Depositing Lab: Marvin Tanenbaum
• Publication: Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001.

Backbone

• Vector backbone: pcDNA4/TO
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: 12xMoonTag-12xSunTag
• Alt name: 12xgp41-12xgcn4
• Promoter: Pcmv

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cgcaaatgggcggtaggcgtg

Gene/Insert 2

• Gene/Insert name: kinesin family member 18b
• Species: H. sapiens (human)
• Entrez Gene: KIF18B

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid has 24 repeats of PP7 hairpins in 3'UTR