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pRSF-1b-S19-T48C
(Plasmid #128597)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128597 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-1b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3669
  • Total vector size (bp) 3945
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    30S ribosomal subunit protein S19
  • Alt name
    rpsS, b3316, ECK3303
  • Species
    E.coli
  • Insert Size (bp)
    279
  • Mutation
    T48C
  • GenBank ID
    NP_417775.1
  • Entrez Gene
    rpsS (a.k.a. b3316, ECK3303)
  • Promoter T7

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF-1b-S19-T48C was a gift from James Williamson (Addgene plasmid # 128597 ; http://n2t.net/addgene:128597 ; RRID:Addgene_128597)

  • For your References section:

    Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding. Duss O, Stepanyuk GA, Puglisi JD, Williamson JR. Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 10.1016/j.cell.2019.10.035. Epub 2019 Nov 21. 10.1016/j.cell.2019.10.035 PubMed 31761533
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