pRSF-1b-S14
(Plasmid
#128596)
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PurposeExpresses tagless 30S ribosomal subunit protein S14 in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSF-1b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3669
- Total vector size (bp) 3972
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name30S ribosomal subunit protein S14
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Alt namerpsN, b3307, ECK3294
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SpeciesE.coli
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Insert Size (bp)303
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GenBank ID
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Entrez GenerpsN (a.k.a. b3307, ECK3294)
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSF-1b-S14 was a gift from James Williamson (Addgene plasmid # 128596 ; http://n2t.net/addgene:128596 ; RRID:Addgene_128596) -
For your References section:
Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding. Duss O, Stepanyuk GA, Puglisi JD, Williamson JR. Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 10.1016/j.cell.2019.10.035. Epub 2019 Nov 21. 10.1016/j.cell.2019.10.035 PubMed 31761533