pLZRS-ER-Rac1-Q61L-shortlinker
(Plasmid #128584)

• Purpose: For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
• Depositing Lab: Julian Downward
• Publication: Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9. doi: 10.1016/j.ccell.2019.05.015. Epub 2019 Jun 27.

Backbone

• Vector backbone: pLZRS
• Backbone manufacturer: Addgene plasmid 21199
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Rac1
• Species: M. musculus (mouse)
• Mutation: Changed Glutamine61 to Leucine in the RAC1 moiety.
• Entrez Gene: Rac1 (a.k.a. D5Ertd559e)
• Tag / Fusion Protein:
  • Modified hormone binding domain of ER alpha (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry