pLZRS-ER-Rac1-P29S-shortlinker
(Plasmid
#128583)
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PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLZRS
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Backbone manufacturerAddgene plasmid 21199
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRac1
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SpeciesM. musculus (mouse)
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MutationChanged Proline29 to Serine in the RAC1 moiety.
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Entrez GeneRac1 (a.k.a. D5Ertd559e)
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Tag
/ Fusion Protein
- Modified hormone binding domain of ER alpha (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLZRS-ER-Rac1-P29S-shortlinker was a gift from Julian Downward (Addgene plasmid # 128583 ; http://n2t.net/addgene:128583 ; RRID:Addgene_128583) -
For your References section:
RAC1(P29S) Induces a Mesenchymal Phenotypic Switch via Serum Response Factor to Promote Melanoma Development and Therapy Resistance. Lionarons DA, Hancock DC, Rana S, East P, Moore C, Murillo MM, Carvalho J, Spencer-Dene B, Herbert E, Stamp G, Damry D, Calado DP, Rosewell I, Fritsch R, Neubig RR, Molina-Arcas M, Downward J. Cancer Cell. 2019 Jul 8;36(1):68-83.e9. doi: 10.1016/j.ccell.2019.05.015. Epub 2019 Jun 27. 10.1016/j.ccell.2019.05.015 PubMed 31257073
