pHalo-MyosinIIB-C25
(Plasmid #128574)

• Purpose: Studies of cellular contractility.
• Depositing Lab: Dylan Burnette
• Publication: Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 10.7554/eLife.42144.

Backbone

• Vector backbone: pEGFP
• Backbone size w/o insert (bp): 3963
• Total vector size (bp): 10863
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myosin IIB
• Alt name: NM_005964.1
• Species: H. sapiens (human)
• Insert Size (bp): 6900
• GenBank ID: NM_005964.1
• Entrez Gene: MYH10 (a.k.a. NMMHC-IIB, NMMHCB)
• Promoter: CMV
• Tag / Fusion Protein:
  • Halo (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • Halo sequencing primers

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry