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human rSHIP2 (18-893)
(Plasmid #128568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrc-HIS
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 7022
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SHIP2
  • Alt name
    SHIP2; 51C protein; INPPL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2622
  • Mutation
    SHIP2 coding sequence from amino acid 18 (A) till amino acid 893 (K) from the EMBL sequence data base Y14385
  • GenBank ID
    NM_001567
  • Entrez Gene
    INPPL1 (a.k.a. OPSMD, SHIP2)
  • Tag / Fusion Protein
    • His Xpress (N-terminal) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pTrc His forward primer
  • 3′ sequencing primer pTrc His reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human rSHIP2 (18-893) was a gift from Christophe Erneux (Addgene plasmid # 128568 ; http://n2t.net/addgene:128568 ; RRID:Addgene_128568)

  • For your References section:

    The SH2 domain containing inositol 5-phosphatase SHIP2 displays phosphatidylinositol 3,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate 5-phosphatase activity. Pesesse X, Moreau C, Drayer AL, Woscholski R, Parker P, Erneux C. FEBS Lett. 1998 Oct 23;437(3):301-3. PubMed 9824312
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