pVenusNfN
(Plasmid #128564)

• Purpose: Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
• Depositing Lab: Oliver Vugrek
• Publication: Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-1111. doi: 10.1177/1087057116659438. Epub 2016 Jul 28.

Backbone

• Vector backbone: pDEST15
• Backbone manufacturer: ThermoFisher Scientific
• Backbone size w/o insert (bp): 7470
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Venus
• Alt name: Split-GFP
• Alt name: Split Venus
• Species: Aequorea victoria
• Insert Size (bp): 474

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Tiago Fleming Outeiro; University Medical Center Göttingen Department of Experimental Neurodegeneration

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)