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Addgene

25Q-ymsfGFP
(Plasmid #128559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS415
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    25Q-Httex1 (without proline-rich region) fused to ymsfGFP
  • Species
    H. sapiens (human)
  • Promoter Gal1
  • Tag / Fusion Protein
    • ymsfGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer Gal
  • 3′ sequencing primer CYC1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    25Q-ymsfGFP was a gift from Patrick Lajoie (Addgene plasmid # 128559 ; http://n2t.net/addgene:128559 ; RRID:Addgene_128559)

  • For your References section:

    Polyglutamine toxicity assays highlight the advantages of mScarlet for imaging in Saccharomyces cerevisiae. Albakri MB, Jiang Y, Genereaux J, Lajoie P. F1000Res. 2018 Aug 10;7:1242. doi: 10.12688/f1000research.15829.2. eCollection 2018. 10.12688/f1000research.15829.2 PubMed 30631438
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