pDONRG_P4-P1R:MUM4_0.3Pro_35S
(Plasmid
#128558)
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Purpose(Empty Backbone) Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepDONRG_P4-P1R
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Backbone manufacturerInvitrogen, modified by 10.5511/plantbiotechnology.11.0124a
- Backbone size (bp) 5892
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Modifications to backbone151 bp within the attR1 sequence there is a thymine to cysteine modification, which does not impact the gateway reaction.
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Vector typeGateway promoter entry clone
- Promoter MUM4 core promoter with 35S enhancer
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor selection use 7 ug/ml Gentamicin
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer 5'-GTAAAACGACGGCCAG-3`
- 3′ sequencing primer 5'-GTAACATCAGAGATTTTGAGACA-3` (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For use with Three-way Multi-site Gateway Cloning (Invitrogen).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONRG_P4-P1R:MUM4_0.3Pro_35S was a gift from George Haughn (Addgene plasmid # 128558 ; http://n2t.net/addgene:128558 ; RRID:Addgene_128558) -
For your References section:
Identification of a seed coat-specific promoter fragment from the Arabidopsis MUCILAGE-MODIFIED4 gene. Dean GH, Jin Z, Shi L, Esfandiari E, McGee R, Nabata K, Lee T, Kunst L, Western TL, Haughn GW. Plant Mol Biol. 2017 Sep;95(1-2):33-50. doi: 10.1007/s11103-017-0631-7. Epub 2017 Jul 20. 10.1007/s11103-017-0631-7 PubMed 28730525
Map uploaded by the depositor.
